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Refolding of synthetic monobodies The polypeptides were dissolved in solubilization buffer (6 M guanidine-HCl, 500 mM arginine-HCl, 20 mM HEPES, pH 8.5, 150 mM NaCl, 0.5 mM TCEP-HCl) at a concentration of 0.5 mg/mL and transferred into a Slide-A-Lyzer G2 dialysis cassette with a cut-off of 3.5 kDa (Thermo Scientific)

Once fully reconstituted, the solution should be clear and colourless

Alternatively, some differences in the established risk factors were observed for fatty liver change, which reveal that TG accumulation progression of NAFLD as risk factors [17]

[CrossRef] [PubMed] [Google Scholar] Melatonin as an antioxidant: Under promises but over delivers

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Look for products that address stability through appropriate packaging, pH buffering and minimal exposure to degradation factors