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[2] Frias JP, Deenadayalan S, Erichsen L, Knop FK, Lingvay I, Macura S, Mathieu C, Pedersen SD, Davies M

Due to the distinct position of the conjugated N-terminal lipid between Cagri-CTR and Cagri-AMY 1 R, we also determined a structure of CTR-Gs in complex with an analogue of Cagri that lacked the lipid modification (CagriBB) to interrogate the contribution of the acylation to the conformation of Cagri-CTR complex

At the time of assay, the growth medium was replaced with HBSS, supplemented with 10 mM HEPES and 0.1% (w/v) ovalbumin and incubated at room temperature for 30 min

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That logic is why its single most advanced research program is not cagrilintide alone but the fixed-dose combination with semaglutide, developed under the name CagriSema

A smaller experimental repeat dataset yielded a similar result with a higher percentage of particles in the bypass (50%) vs the CT-like (25%) conformation