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GDH also plays an important regulatory role in the stimulation of insulin secretion by amino acids (especially leucine)

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Three-micron sections were prepared and mounted on slides, followed by deparaffinization and immunofluorescent staining according to a standard protocol as follows: For deparaffinization, the paraffin-embedded sections were placed at 60 C for at least 30 min, incubated twice in xylene (5 minutes each), and then transferred into 100% ethanol (2 minutes), 100% ethanol (2 minutes), 90% ethanol (1 minute), 80% ethanol (1 minute), and 70% ethanol (1 minute) at room temperature

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Every patient was able to discontinue all immunosuppressive medications, including glucocorticoids, as of final follow-up (median, 15 months)

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