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hiPSCs were cultured on Matrigel (Corning, #356237) in E8 flex medium (Thermo Fisher Scientific) supplemented with puromycin (0.5 g/ml, Sigma Aldrich) and G418 (50 g/ml, Sigma Aldrich) at 37 C/5% CO 2

To use these compounds in research, they must be reconstituted (dissolved) into a liquid solution

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After the reaction, the protein-linker compound was desalted into PBS, and then cyclooctyl group of BCN linker was connected to azide groups of TET linker through copper-free click reaction