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2.5 Liver lipid metabolism-related enzymes activity Liver tissue samples were homogenized (motor-driven Teflon glass homogenizer, Thomas Scientific Swedesboro, NJ, USA) in an ice-cold buffer [9 mM KH 2 PO 4 , 85 mM K 2 HPO 4 , 1 mM DTT, and 70 mM KHCO 3 (pH 7.0)] and centrifuged at 100,000 g for 1 h at 4 C (LE80, Beckman Coulter, Palo Alto, CA, USA)