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In vitro , GA could interacted with natural insulin, inhibiting the key nucleation process essential for the growth of fibrils, thus hindering the formation of amyloid fibers and safeguarding the insulin structure and curtailing protofibril formation (Jayamani and Shanmugam, 2014)

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Ferrostatin-1 (Fer-1) effectively reverses these effects, highlighting its potential therapeutic value (65)

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To investigate whether metformin attenuated MIA-induced OA progression by suppressing oxidative stress, plasma oxidative stress markers including MDA, SOD, and GSH-PX, were measured in the mice (Fig