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After 24 h media was replaced by fresh medium and further incubated for 3 days

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Vitamin B3 has been shown to help skin health, the studies on melasma are limited, however a large trial (Chen, 2017) demonstrated that vitamin B3 (nicotinamide) is an effective and low-risk treatment for non-melanoma skin cancers

[DOI] [PubMed] [Google Scholar] 81.Xu M., Tchkonia T., Ding H., Ogrodnik M., Lubbers E.R., Pirtskhalava T., White T.A., Johnson K.O., Stout M.B., Mezera V., et al