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Briefly, Human GrK and its catalytically inactive mutant GrKSA, in which a serine has been replaced with Ala, were produced in Pichia pastoris and purified using cation-exchange chromatography

5-oxoETE triggers nociception in constipation-predominant irritable bowel syndrome through MAS-related G proteincoupled receptor D

By virtue of the absence of an Fc glycan, AMG 966 is a stable effector functionless (SEFL) antibody, which lacks the ability to interact with Fc receptors (11)