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Membranes were then incubated with horseradish peroxidase (HRP)-conjugated secondary antibody (Cell Signaling Technologies, Cat # sc-516102 and Cat # sc-2357-CM) for 1 h at RT

Histopathological, immunohistochemistry, serum biochemistry, and electron microscopy analyses were performed on rat tissues

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Biochem Biophys Res Commun 436(2):212216 Schenk B, Fulda S (2015) Reactive oxygen species regulate Smac mimetic/TNF-induced necroptotic signaling and cell death [J]

Clonazepam is the most studied of all the agents, although its long-lasting hypnotic and sedative effects might limit its use