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The samples were quickly rinsed with distilled water, differentiated with acidic differentiation solution for 1 min, quickly dehydrated through a graded ethanol series (70, 80, 90 and 100% for 10 sec each), cleared three times with xylene (5 min each), mounted with neutral resin and observed under a microscope

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Moreover, also the core clock component BMAL1 has been reported to bind to p53 promoter, thereby transcriptionally triggering the downstream tumor suppressor pathway 37