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The homogenates were centrifuged (Eppendorf 5810R, Hamburg, Germany) at 1000 g at 4 C for 10 min

141 Another study found that down-regulation of HDAC2 is associated with glucocorticoid resistance

Autophagy inhibition mediated by MCOLN1/TRPML1 suppresses cancer metastasis via regulating a ROS-driven TP53/p53 pathway

CS exposure significantly extended Lm when compared to control group

Expanding beyond canonical cytokine targets, a recent study characterized a novel miRNA ( Rgl-exomiR-7972 ) from Rehmannia glutinosa root vesicles that mitigated LPS-induced lung inflammation in mice by silencing GPR161 , a negative regulator of the Hedgehog signaling pathway

Reactions were initiated by the addition of 10 reaction buffer (250 mM Tris-HCl pH 7.5, 500 mM NaCl, 50 mM MgCl, 50 mM ATP, 5 mM DTT) and incubated at 37 C for the specified durations