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Following incubation, a second centrifugation was carried out at 3000 g for 10 minutes to obtain the supernatant (whey), which was subsequently stored in 10 ml tubes at 20 C for further analysis

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One intriguing recent discovery is that gut microbes can produce small amounts of ammonia and other compounds that affect brain metabolism: a 2025 study showed that manipulating the gut microbiome altered brain amino acid levels and stress susceptibility in mice, partly via microbe-derived ammonia affecting neurotransmitter cycling [88]

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All uncropped and unedited blots are available in the Source Data

Respiratory chain activity and amino acid synthesis Mitochondrial OXPHOS is central to cellular energy production