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mtDNA content was assessed using real-time qPCR on the ThermoFisher QuantStudio3 system, as previously described [27]

Unlike rd10 , which carries a missense mutation in exon 13 of phosphodiesterase 6B ( Pde6b ) 29 , the Rho P23H model carries the p.Pro23His mutation in the rhodopsin gene and shows progressive retinal degeneration similar to that seen in patients with autosomal dominant retinitis pigmentosa 32

TB-500 has been examined in brain injury and neurodegeneration models with research documenting neuronal cell survival promotion through PI3K/Akt anti-apoptotic signalling, promotion of neurogenesis and oligodendrocyte progenitor migration following white matter injury, and anti-inflammatory effects in the injured CNS

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The 10 mg vial is freeze-dried for stability and can be reconstituted with bacteriostatic water to produce a clear solution for in vitro or preclinical use