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Then the cells were centrifuged and washed once with 1 ml of 0.1 M borax (Sigma-Aldrich, 71997), followed by another wash with 1% BSA in PBS

Eight-week-old C57BL/6J mice were randomly assigned to one of four groups ( n = 10 per group): control (CTRL), ethanol (EtOH), ethanol with low-dose NMN (EtOH + NMN(L)), and ethanol with high-dose NMN (EtOH + NMN(H))

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Consistent with H-IOP-induced retinal injury, in the ex vivo Pressure 60 mmHg model, we observed a robust increase in NOX2 immunoreactivity compared to the control, which was specifically localized to the retinal vessels, the ganglion cell layer (GCL) and the inner nuclear layer (INL) (Fig

To view a copy of this licence, visit About this article Cite this article Guan, M., Zheng, X

5 Limitations of current evidence and recommendations for future research The following section discusses the key limitations of existing studies on dietary interventions and oocyte quality, as well as actionable recommendations to address these gaps and advance future research