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The cell pellet was quickly resuspended in a hypotonic lysis buffer (containing 10 mM HEPES (H109408-5g, Aladdin, Shanghai, China), pH 7.9, 1.5 mM MgCl2 (M434105-100g, Aladdin, Shanghai, China), 10 mM KCl (P433501-250g, Aladdin, Shanghai, China), 0.5 mM DTT (D301909-1ml, Aladdin, Shanghai, China), and washed with it