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Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

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Further studies are required to determine how CaMKII, Rho GTPases, and associated GEFs and GAPs participate in spine formation/elimination under physiological learning conditions in vivo

Tesofensine, a novel antiobesity drug, silences GABAergic hypothalamic neurons

They shorten every time a cell divides, until they become too short to allow proper cell division