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RNA concentration, purity, and integrity were evaluated with a Qubit 2.0 Fluorometer (Life Technologies, Q33216, USA) and the Qubit RNA Assay Kit (Shanghai Baoji Biotechnology, HKR2106-01, China), a NanoDrop spectrophotometer (IMPLEN, USA), and the RNA Nano 6000 Kit on the Agilent Bioanalyzer 2100 system (Agilent, 5067-1511, USA)