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Incubation with primary antibodies (1:1000) for rabbit anti-HO-1(Abcam, UK), anti-IL-6 (Abcam, UK), rabbit anti anus kinase 2 (Jak2) (# 3230S, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-pY1007/1008 Jak2 (# 3776S, Cell Signaling Technology, Danvers, MA, USA), rabbit anti- signal transducer and activator of transcription 3 (STAT3) (# 12640, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-pY705 STAT3 (# 9145S, Cell Signaling Technology, Danvers, MA, USA) and -actin overnight at 4C was done

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