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Proteins (30 g) underwent electrophoresis on 12% SDS-PAGE gels, transferred onto PVDF membranes (Millipore, United States), and blocked with QuickBlock Blocking Buffer (Beyotime, P0252) for 1 h

10.1155/2020/5849794 104 WangY

This acetylation increases the lipophilicity of the molecule, which has two important consequences

Clinical trials guiding evidence for treatment are lacking due to the rarity of disease, heterogeneous clinical course, and lack of prognostic biomarkers, all of which contribute to difficulty in clinical trial design and implementation

After incubating for 24 h at 37 C, the medium was collected, and formaldehyde was quantified