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DNA dot-blot analysis Genomic DNA from erythroblasts was extracted using phenol:chloroform:isoamyl alcohol (25:24:1) and precipitated with ethanol before dissolving in water

Perry, D

U-87 MG cells were harvested from a human glioblastoma tumor, and although it is in their inherent nature to cluster when they are cultured in standard 2D culture, they adopt fibroblast-like morphology and only make small aggregates (Fig 1A)

We will also perform Bugbuster protein extraction followed by SDS-PAGE to assess the level of expression for the three glutathione enzymes

Endocrinology 136: 421430, 1995 [DOI] [PubMed] [Google Scholar] 121.Huang C, Freake HC

The emerging diversity of the lysine acylation landscape prompted us to investigate the function and mechanism of these novel HPTMs in health and disease