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Pathophysiology of ischemic stroke: role of oxidative stress

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Superoxide reporters of the DHE family yield non-specific raw fluorescence, thus quantification must rely on chromatographic separation of DHE2-hydroxyethidium (2-OH-E) by HPLC to measure O 2 and discriminate it from ethidium or other adducts, ideally alongside SOD controls and pathway-selective perturbations (e.g., NOX2 inhibitors, mitochondrial site inhibitors) for source assignment

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Methods 3 (5), 357359

After another 48 h, medium was collected and centrifuged at 500 g for 10 min to pellet debris