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Although hearing loss, particularly cochlear or other forms of peripheral auditory damage, remains a major risk factor, it does not fully account for the persistence of the condition, as evidenced by its continuation following auditory nerve transection 86 and its occurrence in individuals without measurable hearing loss

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Then, after rinsing in PBS, the primary antibodies were revealed by applying the appropriate secondary FITC-conjugated antibodies (AlexaFluor 488, 1:500) for 1 h at room temperature

J Clin Exp Hepatol 12(4):11501174

Tapio, I