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Staining results from both cells and tissues were observed and imaged using an Axio Observer 7 microscope (Carl Zeiss Meditec AG, Jena, Germany)

For cDNA plasmid transfection, cells were plated in a 24-well plate and transfected using LipoD293 (Signa Gen Laboratories) with a total of 500 ng of plasmid DNA per well

Bee venom: overview of main compounds and bioactivities for therapeutic interests

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