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TMAO and cardiovascular disease The connection between TMAO and CVD was first made using an animal model in 2011, which revealed that choline, TMAO and betaine were predictors of CVD risk

Couillard C, Pomerleau S, Ruel G, Archer WR, Bergeron J, Couture P, et al

Uch-L1 C-terminal hydrolase activity can be measured in vitro by an enzyme assay using the fluorogenic substrate Ub-AMC, and its activity can be effectively inhibited by the specific inhibitor LDN-57444 (Wilkinson et al., 1989