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(2016) Selenium inhibits Staphylococcus aureus -induced inflammation by suppressing the activation of the NF- B and MAPK signalling pathways in RAW264.7 macrophages

Antibodies and reagents Primary antibodies used were anti-GPX4 (1:1000 for WB, A11243, Abclonal), anti-CBS (1:1000 for WB, A11612, Abclonal), anti-CTH (1:1000 for WB, A6121, Abclonal), anti-SCLY (1:1000 for WB, A15842, Abclonal), anti-GAPDH (1:1000 for WB, A19056, Abclonal), and anti-Actin (1:1000 for WB, HC201-01, TransGen)

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Briefly, 4.5 g of GSH and 1.0 g of NaNO were dissolved in 23 mL of deionized water containing 1 mL of concentrated hydrochloric acid (37%, w/w) and ethylenediaminetetraacetic acid, EDTA (100 M) added as a chelating agent