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Western blotting Cells were washed, lysed in IP-lysis buffer (30 mM Tris-HCl, 120 mM NaCl, 2 mM EDTA, 2 mM KCl, 1% Triton-X-100, pH 7.4, protease and phosphatase inhibitor (Roche)) and frozen at 20 C

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Glutathione elicitation of isoflavan phytoalexins in transformed root cultures

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