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Therefore, the effects of the developed system on cells were evaluated using a human fibroblast cell line (HFD)

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The cells were cultured in Eagles minimum essential medium (ThermoFisher Scientific) or DMEM F-12 (ThermoFisher Scientific) respectively, supplemented with 10% Fetal Bovine Serum (Gibco) and 1% penicillinstreptomycin (ThermoFisher Scientific), in a humidified incubator maintaining 5% CO 2 at 37 C

Unless we are malnourished, we arent truly deficient in this substance, but there are many benefits to increasing our intake of the nutrient

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