affinity purification of a glutathione-s-transferase fusion protein Schematic outline the GST-His two-step method GST-tagged Protein Purification Kit

M., Bhavsar, V., Tripoli, G

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Cys 2 ) and cystine-free (Cys 2 -free) medium with vehicle (DMSO) or RSL3 (250 nM), alone or with Fer-1 (10 M) or Trolox (100 M)

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Thereafter, trypsin at 1:50 was poured for overnight digestion and that at 1:100 was introduced for additional 4 h of digestion, followed by peptide desalting using a C18 SPE column

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