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Intracellular GSH levels A2780 and A2780DDP cells were incubated for up to 24 h with 50 M of buthionine sulphoximine (BSO), which inhibits the GSH synthesis, and the intracellular GSH was measured (Figure 3)

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Then the pellets were stained for 20 min in 100 l assay buffer containing 5 g ml 1 propidium iodide (Sigma-Aldrich, P4170) and 3 l Annexin VFITC (BioLegend, 0640906)

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In HCECs applied with desiccating stress, the CEC ferroptosis was triggered by the aggregation of autophagy impairment-derived sequestosome 1 (SQSTM1), the overexpression of which fostered ferroptotic changes and substantially elevated ACSL4 (124)

Cell Death Differentiation 18, 12471256