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[Wiley] 131

A denaturing agent, such as 8M urea, will also break the interaction by altering the configuration of the site where the antigen binds molecule of the antibody 4 Step : Affinity medium is re-equilibrated with binding buffer

Gln81 and Trp136 function to stabilize Sec46, especially the selenium redox intermediates (Se-, SeO-) through a network of hydrogen bonds

PMC 4417967

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