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doi:10.1016/j.lfs.2004.04.038

Hypocretin (orexin) cell loss in Parkinsons disease

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A 6 L sample of this mixture was then loaded onto a 16% denaturing polyacrylamide gel (containing 20% formamide in TBE buffer) and subjected to electrophoresis at 500 V for 90100 min at room temperature