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Studies by Pickart and colleagues published in 2012-2018 showed that GHK-Cu treatment of cultured human fibroblasts altered expression of several thousand genes, with patterns indicating increased expression of genes involved in tissue repair, antioxidant responses, protein synthesis, and cellular rejuvenation

The resulting solution was incubated at 25 C for 30 min, and absorbance was measured at 765 nm

View Article Wound Repair and Regeneration (2008) "The effect of GHK-Cu on wound healing and scar formation." View Article International Journal of Molecular Sciences (2018) "Regenerative and Protective Actions of the GHK-Cu Peptide in the Light of the New Gene Data." View Article

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We've found that -20C is perfectly adequate for short to medium-term storage (up to a year or two), while -80C extends its viability considerably, often for several years

(2003) investigated Epitalon in human fetal fibroblast cultures that had reached replicative senescence