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Large-scale randomized controlled trials (RCTs) are needed to confirm the long-term safety and efficacy of these compounds, particularly in preventing or mitigating age-related diseases like Alzheimers disease, osteoarthritis, and cardiovascular disorders.123 Most current evidence comes from short-term studies or observational data, which makes it difficult to determine the real-world, long-term effects.191 Translating Preclinical Findings to Humans: While animal models have shown encouraging results, the translation of these therapies to humans remains challenging.192 Species differences, variations in the aging process, and the complexity of human aging may complicate the successful application of senomorphic agents in clinical practice.193 Therefore, further clinical trials are needed to validate the efficacy and safety of these agents in human populations

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Total renal and aortic Gstm1 expression in transgenic animals was significantly increased compared with SHRSP [renal relative quantification (RQ): SHRSP-Tg(Gstm1)1WKY 1.95 vs