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0.5 ml 0.1%, trichloroacetic acid (TCA), leaf extract supernatant, 0.5 ml of 100 mM potassium phosphate buffer, and 2 ml reagent (1 ml KI w/v double-distilled water) were added in the reaction mixture

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The Scientific World Journal Volume 2014 (2014), Article ID 417842, 10 pages Reactive Carbonyl Species In Vivo: Generation and Dual Biological Effects Mitochondrial oxidative stress caused by SOD2 deficency promotes cellular senescence and aging phenotypes in the skin