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Briefly, samples were mixed with Tris-EDA-HCl buffer and pyrogallol (1:15:1) and incubated at 25 C for 10 min

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The FOXO1 (5DUI) was found to have hydrophobic, hydrogen, and polar amino acid interactions with SER 184, GLN 185, GLU 188, LYS 192, and LYS 233