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The recombinant strains were cultured to an OD 600 of 0.6 and induced with 0.5 mM IPTG for 12 h at 25 C

10.1021/tx960072x

Cloning and Characterization of the Gene Cluster for Biosynthesis of Ectoine from Nesterenkonia Halobia DSM 20541

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& Zajac, A