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We performed densitometry analysis to calculate the intensity of phosphorylated and total protein using ImageJ software

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Also, activation of liver GH receptor, promotes IGF-I synthesis which, in turn, is released to the circulation and can be found in its free form but mainly bound to IGFBPs (overall IGFBP-3, which binds ~90% of circulating IGF-I)[23]

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Moreover, although preclinical studies have yielded promising results, large-scale, double-blinded clinical trials remain necessary to verify the therapeutic efficacy and feasibility of these approaches in DN patients

In melanoma and breast cancer mouse models, it has been observed that mice fed a low-protein diet (4% kcal protein) exhibit reduced IGF-1 levels and slower tumor progression compared to those fed a high-protein diet (18% kcal protein)