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Jerne explained the rationale behind his method in his diary in March 1962 as follows: 'Antibody molecules diffusing from a single cell into agar containing red cells and complement should lyse [puncture] a sufficient number of surrounding red cells to produce a visible plaque'(Jerne, 1981)

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Single-cell transcriptome analysis defines heterogeneity of the murine pancreatic ductal tree

Community protocols using 2-5 mg/kg in humans are deliberate choices by early adopters, not FDA-guided conversions

Henne WM (2019) Spastin joins LDs and peroxisomes in the interorganelle contact ballet

Signs of Degradation Don't use the reconstituted solution if it appears cloudy, contains visible particles that don't dissolve with gentle swirling, or has changed color