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Unlike older peptides like GHRP-6 or GHRP-2, it does not cause a massive spike in ghrelin (the hunger hormone) that leads to overeating

Gastroenterology 123 , 163172 (2002)

Neuropsychiatry Neuropsychol Behav Neurol

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Western blot analysis The levels of cellular proteins were quantified using Western blot analysis by utilizing primary antibodies: anti--catenin (1:1000), anti-pGSK3 (Ser9, 1:1000), anti- tGSK3 (1:1000), anti-cyclin D1 (1:1000), anti-p-Akt (1:1000), anti-tAkt (1:1000) (all from Cell Signaling Technology, Danvers, MA, USA), or anti-GAPDH (1:5000