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After anesthetizing C57BL/6 mice with 2% pentobarbital, the midline incision was taken to expose the bladder position of the mouse

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In contrast, Europe and North America have reported lower contamination levels, thanks to stringent regulatory frameworks, but sporadic outbreaks continue to occur, particularly with ochratoxins in grains and T-2 toxins in improperly stored cereals [242]

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As a result of this process, known as repeat-induced point mutation (RIP), the wild-type Neurospora genome contains a small fraction of methylated DNA, the majority of the DNA remaining nonmethylated