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Each sample was then split into three fractions (100 L) and treated as follows: For H 2 S measurement, 100 L sample was precipitated with 1 mL of absolute MeOH at 40 C for 2 h, centrifuged (16,000 g ), and obtained supernatant was evaporated and the residue was resuspended and sonicated in 100 L of 50 mM HEPES buffer (pH 7.4)

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In animal studies, BPC-157 has been administered via injection (into the abdomen, into muscle, or directly into tissue), orally (in drinking water or by mouth), and topically (as a cream)