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ERK1/2 phosphorylation displayed a biphasic kinetics showing a peak at 5 min, followed by a decline and a weaker second peak at 30 min in IR and IR/IGF1R-expressing cells (Supplementary Fig

In the lysosomal lumen, arginine and leucine facilitate the activation of Ragulator by stimulating SLC38A, a sodium-coupled amino acid transporter and lysosomal V-ATPase on the lysosomal membrane, thereby promoting the binding of Rag GTPases and mTORC1 62,63,64

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As kidney function deteriorates, these toxins accumulate in the blood, causing many deleterious effects on body tissues and organs, thereby contributing to kidney damage and related complications such as cardiovascular disease and neurological disorders (Krukowski et al., 2023

It is still unknown whether long-term supplementation with NAD + precursors has any side effects