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In ELISAs the antibody of interest does not have to be conjugated to a detectable compound

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Also, -carotene supplementation was found to increase the risk of lung cancer in smokers regardless of the nicotine and tar content of cigarettes, indicating that smokers should refrain from taking this vitamin as a supplement unless they have vitamin A deficiency

If you have already used or are currently using either of these compounds, it is important to disclose this to your GP or pharmacist without delay