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New York Times

ASO2MT44, Life Technologies Corporation) were dissolved in citrate buffer (25 mmol l 1 , pH 4.0)

1f), indicating a lack of cytosolic cargo proteins

A second nested-PCR enrichment step was performed for 11 cycles using the same cycling parameters as before, using the entire bead-purified reaction product from the first step as a template with primers YL009 (CAAGCAGAAGACGGCATACGAG) and JBD-SS-273:280 (AATGATACGGCGACCACCGAGATCTACAC TATAGCCT ACACTCTTTCCCTACACGACGCTCTTCCGATCT NNNNNNN GCAGGGATGTCCACGAGGTCTCT) where N 8 barcodes correspond to reverse-complemented Illumina TruSeq HT D501-508 indexes