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Animals were euthanized under general anesthesia (overdose of pentobarbital) at the termination of each study

Proper injection technique and site rotation can minimize these effects

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For homologous recombination the CEN and URA fragments along with the SEO1 gene fragment, carrying vector overlapping sequences, were transformed in yeast using lithium acetate method and plated on SD-URA plates

Since some SNPs reduce methylation while others increase it, a patients overall methylation status depends on the combined impact of these SNPs