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Images from H&E-stained sections were acquired using an Axiovert 200M microscope with an Axiocam HRc camera and Axiovision software (Carl Zeiss)

Data from two groups were compared using two-tailed unpaired t -tests for unpaired experiments, or paired t -tests for self-control mouse experiments

The distinct mode of CTR and AMYR engagement by Cagri revealed in the current study, together with the correlated distinctions in functional responses at individual receptors, is likely to be important in the differentiated, high efficacy, of Cagri as a first-in-class obesity drug in both preclinical animal models and in treatment of obese patients in clinical trials 24

Translocator protein/peripheral benzodiazepine receptor is not required for steroid hormone biosynthesis

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