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Based on different bleeding sites, hemorrhagic stroke can be generally divided into intracerebral hemorrhage (ICH) and subarachnoid hemorrhage (SAH), whose pathogenesis share some similarity

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Immunoprecipitation was performed with Flag agarose M2 affinity beads (Sigma-Aldrich, A2220, 15 l per sample) or with BUD13 antibody (A303-321A) prebound to protein G Dynabeads beads (20 l per sample)

Besides this, dockings were performed using default parameters

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