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10.34172/mejdd.2021.203 114 TanakaK.NakamuraS.NarimatsuH

Aliquots at the various time points after fecal slurry inoculation were collected and acidified with 2 mL 0.1N HCl (Sigma-Aldrich, 50%/50% v/v), centrifuged at 4 C at 900 g for 8 min, filter-sterilized in a 0.22-m cellulose acetate filter, and frozen at 80 C until performing the experiments for the determination of the amount of TMA in the fecal slurry supernatants

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Other tests have demonstrated that naringin, paeoniflorin, -ecdysterone, 18-glycyrrhizic acid, amygdalin, albiflorin, and saikosaponin A can downregulate FMO3 activity and reduce TMAO biosynthesis (85)

Central role of dysregulation of TGF-/Smad in CKD progression and potential targets of its treatment