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Biological evaluations were conducted using human hepatocarcinoma cell line (HepG2 cells) under two conditions: glucose-containing and glucose-free (galactose-supplemented) media to model mitochondrial toxicity

Oxidative stress from superoxide production was determined by DHE in sections from all groups in the presence or absence of the inhibitors L-NAME or PEGSOD and the fluorescence quantified by automated image analysis

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Although endogenous synthesis partially compensates, supplementation may be relevant, particularly for vegan athletes, vegetarian seniors, or during caloric restriction periods

Chao OY, Pathak SS, Zhang H, Dunaway N, Li J-S, Mattern C, et al