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Mice were maintained on a standard diet, and water was freely available

Splitting vials across titration phases Some researchers reconstitute their 10 mg vial at a higher concentration (5 mg/mL with 2 mL water) for the later phases when precision is less critical and injection volume matters more

A conservative approach using 0.25 mg daily for the first two weeks allows assessment of individual response before considering increases

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Large meals overwhelm a stomach that is emptying more slowly due to GLP-1 activation